Despite the acute, resolving nature of many parvovirus infections, it is now well established that many members of the family Parvoviridae, such as the human erythrovirus
B 19, can establish lifelong persistence with restricted replication and absence or rarity of detectable long-term viremia (5-7).
Until recently, parvovirus B19 and adeno-associated viruses, which belong to the genera Erythrovirus
and Dependovirus, respectively, were the only known members of the family Parvoviridae that infected humans (1).
Phylogenetic analyses of sequence data suggest that this virus, termed PARV4, is only distantly related to previously known human or animal members of the family Parvoviridae, including members of the Erythrovirus genus known to infect humans, such as parvovirus B19.
The specificity of these primers was confirmed by PCR using a cloned fragment of the ORF1 region alongside erythrovirus control material (Figure 1A).
Plasma pools found positive for PARV4 sequences were tested for the levels of erythrovirus DNA as described previously (4).
Before such measures were introduced, more than half of production start pools contained erythrovirus DNA, some with titers of [10.
Identification and characterization of a second novel human erythrovirus variant, A6.
Rhesus and pigtailed macaque parvoviruses: identification of two new members of the erythrovirus genus in monkeys.