However, high ethanol and levan production was obtained with recycle fermentation in Erlenmeyer flasks
and with the reuse of immobilized support by semi-continuous process in the fermenter.
A single fresh colony of the growth was activated in a 50 mL Falcon conical tube (BD Biosciences) containing 5 mL of tryptic soy broth at 37oC for 12 h on a rotary shaker (220 rpm) and 1% of the 12 h culture was then transferred into 250 mL Erlenmeyer flask
containing 50 mL of the medium followed by incubation at 37oC for 24 h.
45 [micro]m pore diameter) to be added under aseptic conditions to 250 mL Erlenmeyer flask
containing 45 mL sterile medium.
a) Shake flask: Calcium gluconate fermentation was carried out by submerged fermentation in 250 ml cotton plugged Erlenmeyer flasks
containing 25 ml of fermentation medium.
It was diluted to the required concentrations of dye (50 ppm -150 ppm) in 250 ml Erlenmeyer flasks
by mixing with appropriate volumes of deionized water.
The 750 ml Erlenmeyer flasks
containing 70 ml of complex nutrient medium were incubated at 30[degrees]C in a rotary shaker Innova 43 (New Brunswick Scientific Co.
This year, 13 schools each received about $3,000 worth of laboratory products including pipettes, centrifuge tubes, beakers, media bottles, graduated cylinders, Erlenmeyer flasks
, dishes and hotplates/stirrers.
SSF was conducted in 250 ml Erlenmeyer flasks
A volume of 100 mL of NBC was dispensed in Erlenmeyer flasks
, which were inoculated with 1.
containing 100 ml M1 medium were inoculated with an aliquot of the concentrated spore's suspension in order to obtain 5x[10.
A loop-full of cells from the respective stock cultures were incubated into 100ml sterile nutrient broth contained in 250ml Erlenmeyer flasks
Testing of different sorts of fungi for the production of pectolytic enzymes was performed in 500 ml Erlenmeyer flasks
(with 100 ml nourishing base) on a laboratory rotational shaker with rotational shaking (200 rev/min), at a temperature of 30[degrees]C within 48 hours.