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endonuclease |
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endonuclease /en·do·nu·cle·ase/ (-noo´kle-ās) any nuclease specifically catalyzing the hydrolysis of interior bonds of ribonucleotide or deoxyribonucleotide chains. restriction endonuclease an endonuclease that hydrolyzes DNA, cleaving it at an individual site of a specific base pattern.
endonuclease, an enzyme that cleaves or hydrolyzes phosphodiester bonds within a polynucleotide chain. Compare exonuclease. endonuclease (en´dōnoo´klēās´), n an enzyme (nuclease) that cleaves polynucleotides at interior bonds, producing polynucleotide or oligonucleotide fragments. endonuclease a nuclease that cleaves internal bonds of polynucleotides. restriction endonuclease How to thank TFD for its existence? Tell a friend about us, add a link to this page, add the site to iGoogle, or visit webmaster's page for free fun content. |
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For pulsed-field gel electrophoresis (PFGE), bacterial plugs were digested with restriction endonucleases XbaI and SpeI. The PCR assay was combined with a restriction endonucleases digestion (Bsg I) of the amplification products to differentiate between different forms of Hematodinium from different hosts. To be considered a match, the isolates from two or more patients required complete agreement of all bands by PFGE after digestion with two restriction endonucleases. |
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