However, a modified Elek test yielded a negative result (8).
ulcerans, suggesting a higher sensitivity of cytotoxicity assays than that seen with the Elek test (15).
We determined DT production using a modified Elek test (6); we used C.
All isolates were nontoxigenic tox-bearing (NTTB) strains as shown by positive tox-PCR, and negative Elek test and Vero cell cytotoxicity results.
A modified Elek test for detection of toxigenic corynebacteria in the diagnostic laboratory.
FRC24 is a toxigenic isolate; toxigenicity was confirmed by both tox gene detection and Elek test
Results from use of the modified Elek test
(6) indicated that all feline isolates were negative for production of diphtheria toxin; however, an atypical precipitation was observed after 36 h of incubation.
ulcerans tox-specific PCR (4), and the Elek test
as described previously (4,5).
Toxigenicity was determined by both dtx polymerase chain reaction and Elek test
Easy-to-perform modified Elek test
to identify Shiga-like toxin-producing diarrhoeogenic Escherichia colt.
Toxigenicity status was determined by the Elek test, as recommended by WHO (9), and by the polymerase chain reaction (PCR), which targeted both A and B subunits of the tox gene (10).
No discrepancies between the results obtained by traditional identification, the API Coryne test, or toxigenicity testing by the Elek test and PCR were detected.