Conventional methods for detecting DNA-binding proteins are electrophoretic mobility shift assay
(EMSA)  (7), DNA footprinting (8), ELISA (9), and Southwestern blotting (10).
NF-[kappa]B activation assay, electrophoretic mobility shift assay
(EMSA) was performed as previously published (Madan et al.
Since topo II antibody binding was inconclusive and suitable antibody controls were not available, we took a more direct approach of using the purified topo II protein in the electrophoretic mobility shift assay
We performed electrophoretic mobility shift assays
(EMSAs) by use of the LightShift Chemiluminescent EMSA reagent set (Pierce) on nuclear extract of HepG2 cells.
The repression of E-selectin mRNA expression caused by the pentacyclic triterpenoid acids paralleled the inhibition of NF-kB activation and nuclear translocation, as evaluated by electrophoretic mobility shift assays
, although the degree of repression by UA was approximately two times more effective than that of OA.