EcoR1

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EcoR1

EcoR1 restriction endonuclease.
References in periodicals archive ?
We were unable to map more than three or four alpha-tubulin loci because only four loci were contained in restriction fragments polymorphic in the two parents in HindlII and EcoRI digests.
5-1 [micro]g) was digested by restriction enzymes EcoRI and MseI for 1 h at 37[degrees]C.
The pre-amplification DNA was diluted 5-fold with ddH2O and selective amplifications were carried out by using different EcoRI and Tru9' primer combination (Alpha DNA).
Eight Msel and EcoRI selective primers with sixty-four primer combinations were tested.
Ten micrograms of high-molecular-weight DNA was digested with 200 units of EcoRI, HindIII, and BamHI (Promega, Madison, Wis) in the presence of 4 mmol/L spermidine (Sigma) and 100 mg/mL bovine serum albumin (Sigma) for 3 to 4 hours, precipitated in ethanol, and reconstituted in TE buffer (10 mmol/L Tris-HCl, pH 7.
380 was generated; two restriction sites, EcoRI and NheI, were found to flank the promoter sequence.
Seven clones with inserts of the expected size were identified after EcoRI and HindIII digestion.
Plasmid Miniprep Kit (Omega, Norcross, GA, USA) and identified by EcoRI restriction digestion (Promega).
Both strains were made self-bioluminescent by transformation, using standard methods previously described (9), with the broad-host vector pBBR1MCS-2 (17) carrying the luxCDABE cassette from Photorhabdus luminescens as an EcoRI PCR fragment.
EcoRI primer Mse I primer Total No selective selective number of nucleotides nucleotides bands 1.
Each amplification product was digested by EcoRI, and restriction profiles were observed by gel electrophoresis.