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EcoR1 |
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EcoR1 EcoR1 restriction endonuclease. How to thank TFD for its existence? Tell a friend about us, add a link to this page, add the site to iGoogle, or visit webmaster's page for free fun content. |
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| EcoRI (Promega, Madison, WI) was used to digest plasmids to confirm whether the plasmids harbored PCR products, isolated plasmids were digested overnight with 11 [micro]L EcoRI, 1 [micro]L 10X buffer, 6 [micro]L deionized sterile water, and 2 [micro]L plasmid DNA at 37[degrees]C. Restricted by the endonuclease EcoRI, the 18 transferred plasmids produced 9 major restriction patterns (Figure 1 and Table 2). For construction of the Gal4 DBD-PXR ligand binding domain (LBD) fusion plasmid (Gal-PXR), PCR was used to amplify LBD-specific fragments of rat PXR; exonuclease III-mediated subcloning (Li and Evans 1997) was used to clone the fragments between the EcoRI and BamHI sites of pCMX-Gal4. |
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