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EcoR1
(redirected from EcoRI)

   Also found in: Wikipedia 0.04 sec.
EcoR1
EcoR1 restriction endonuclease.


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EcoRI (Promega, Madison, WI) was used to digest plasmids to confirm whether the plasmids harbored PCR products, isolated plasmids were digested overnight with 11 [micro]L EcoRI, 1 [micro]L 10X buffer, 6 [micro]L deionized sterile water, and 2 [micro]L plasmid DNA at 37[degrees]C.
Restricted by the endonuclease EcoRI, the 18 transferred plasmids produced 9 major restriction patterns (Figure 1 and Table 2).
For construction of the Gal4 DBD-PXR ligand binding domain (LBD) fusion plasmid (Gal-PXR), PCR was used to amplify LBD-specific fragments of rat PXR; exonuclease III-mediated subcloning (Li and Evans 1997) was used to clone the fragments between the EcoRI and BamHI sites of pCMX-Gal4.
 
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