The funding round included participation from leading biotechnology and other investors: The Column Group, Topspin Partners, OrbiMed Advisors, EcoR1
Capital, Foresite Capital and Kravis Investment Partners.
The financing was led by new US based investors Vivo Capital and OrbiMed, and includes EcoR1
Capital, Boxer Capital of Tavistock Life Sciences and new investment from existing investor HBM.
Among the new investors in the funding round were Cowen Group, EcoR1
Capital, Goudy Park Capital, Portola Capital Partners and Sabby Capital.
Oligonucleotides Purpose Sequence (5'-3') EcoR1
-forward adapter Adapter ligation CTCGTAGACTGCGTACC EcoR1
-reverse adapter Adapter ligation AATTGGTACGCAGTCTAC Mse 1-forward adapter Adapter ligation GACGATGAGTCCTGAG Mse 1-reverse adapter Adapter ligation TACTCAGGACTCAT EcoR1
primer Pre-amplification GACTGCGTACCAATTC Mse1 primer Pre-amplification GATGAGTCCTGAGTAA E-ACA Selective amplification GACTGCGTACCAATTC + ACA E-ACT Selective amplification GACTGCGTACCAATTC + ACT M-CTA Selective amplification GATGAGTCCTGAGTAA + CTA M-CTG Selective amplification GATGAGTCCTGAGTAA + CTG TABLE 3.
by PCR amplification of spacer sequences separating a subset of copia-like EcoR1
Restriction enzymes EcoR1
, BamH1, Hind III were from New England Biolabs (Boston, MA, USA), AluI, HaeIII, Sau3A were from Promega ((Madison, WI, USA)
12) This study did report a significant association with an EcoR1
RFLP located in exon 29 which is identical to SNP rs1042031.
PCR products were digested using restriction enzymes BSeG 1, EcoR1
Cell clones were expanded into two 100 mm culture dishes; genomic DNA was extracted from one dish, and at least 10 [micro]g DNA from each clone was digested with EcoR1
overnight at 37[degrees]C, and subsequently separated on a 0.
On the other hand, the digestion of the plasmid using restriction enzymes such as the EcoR1
and Xho1 was used to determine the size of the inserted gene and to confirm that the expected gene was inside the plasmid.
The set of primers were PALFor1 EcoR1
and BamH1 are two enzymes that can be used to cut pBr322 and will yield 375 bp and 3987 bp fragments.