Linear regression analyses of all 3 ETEC
qRT-PCR assays resulted in an estimated LOD at 30 CFU per 20 [micro]L of reaction volume and 3 CFU per 20 [micro]L reaction volume for Shig-ipaH qRT-PCR assay (Table 1).
strains were isolated from children with diarrhea.
iii) The discovery of pili (fimbria) in ETEC
Sequencing of the same region (performed as described ) in the 24 ST-only isolates in this study showed that all STp/CS6 isolates had identical CS6 sequences to the STp/CS6 ETEC
strain GB124 (GenBank accession no.
A computer-based search was performed in Medline to retrieve scientific literature published between 1970 and July 1999 using the key words: ETEC
, Escherichia coli, diarrhea, human, morbidity, and mortality, which yielded 3,302 articles, of which 664 were culled.
(serotype O6:NM) that produced LT and ST was isolated from stool specimens from seven of nine ill guests and from one of five well employees.
strains were isolated from acute watery non-bloody stool samples obtained from hospitalized children with diarrhea under five years of age in La Paz, Bolivia, between 2002-2006.
Virulence factors and identification of protective antigens in ETEC
For ciprofloxacin, ETEC
strains demonstrated a ninefold increase in resistance, from 3% in 1997 to 20% in 2006-2008.
The most common agent in all regions was ETEC
, with 270 samples isolated from the total 291 travelers to Mexico/Guatemala (grouped together as "Latin America") and 98 of 143 travelers to India.
To broaden his skills, he enrolled on a series of Learning and Skills Council (LSC) North East funded courses with ETEC
Ltd, a training and development company based in Sunderland.
uses thin hair-like fibers called "pili" or "fimbriae" on its surface to attach to host intestinal epithelia, an early, vital step in diarrhea pathogenesis.