Of the 50 EHEC
strains examined (Table), 48 were serotype O157:H7/H- and 2 were O55:H7.
In addition, the Centers for Disease Control and Prevention (CDC) in Atlanta, United States of America have published information on 3 HUS cases (1 confirmed and 2 suspected) and 1 suspected EHEC
case (without HUS) in the United States linked to this outbreak.
Subsequent LightCycler([R]) 480 Real-Time PCR was found to be accurate and reproducible using proven specific, sensitive PCR primers and probes, targeting the Shiga toxin genes of EHEC
, available as LightMix([R]) kit EHEC
However, unlike EHEC
serogroup O157, it may be pathogenic for both cattle and humans (17).
PCR was performed individually using the above primer pair for stx1, stx2, rfb O157, EHEC
hlyA and eaeA genes.
coli strain from Europe is detected by Meridian's shiga toxin-detecting assays, Premier([TM]) EHEC
and ImmunoCard STAT
We collected 278 eae-positive strains that were originally identified by routine diagnostic protocols as EPEC or EHEC
As of 05 June at 10:00 CET, 1536 cases of EHEC
infection (without HUS) had been reprted in Germany (with 6 fatalities): 108 more than the previous day.
PENZBERG, Germany -- With the recent outbreak in Europe of Enterohaemorrhagic Escherichia coli (EHEC
), Roche Applied Science (SIX: RO, ROG; OTCQX: RHHBY) and TIB Molbiol are providing scientists with complementary technologies that can be used for the rapid identification and detection of EHEC
28 Brucellosis 699 225-1,378 Campylobacteriosis 3,571# 851-7,733 EHEC
coli O104:H4 strain associated with the current EHEC
outbreak in Europe.
pO113 was isolated from another hemolysin-producing EHEC