In summary, we describe the isolation of EHDV
that is involved in hemorrhagic disease affecting pygmy brocket deer in Brazil.
A competitive ELISA provided by the Institute of Animal Health (Pirbright, UK) was performed to detect IgG against EHDV according to the protocol of Thevasagayam et al.
Antibodies against EHDV were detected in 9% of the cattle tested.
Our results indicated that BTV and EHDV were widespread in Algeria.
Seropositivity for BTV and EHDV in livestock in Algeria, 2008 * No.
Our results provide serologic indication that EHDV
and BTV circulate in Rusa deer in Mauritius.
These 6 samples and the 1 original positive cell culture were further tested by serotype-specific RT-PCRs that targeted segment 2 for identification of EHDV serotype.
Of the selected samples submitted for BTV and EHDV testing, the positive identification of EHDV RNA supports initial clinical identification of an EHD outbreak in Turkey.
That only 1 of the 4 original serum samples was positive for EHDV antibodies by ELISA can be explained by time of sample collection.
Depending on the segment, TOV was placed in a different neighborhood among BTV and EHDV
(Figures 2, 3).