The impact of tagged single-nucleotide polymorphisms (tagSNPs) and inferred haplotypes in 12 candidate genes (ACE, ADRB2, AGT, AGTR1, ALOX15, EDN1, GRK4, PTGS1, PTGS2, TLR4, VEGFA, and VEGFB) on the relationship between residential proximity to major roadways and LVM was analyzed using multiple linear regression, adjusting for multiple potential confounders.
Twelve genes were selected from among genotyped MESA candidate genes: ACE, ADRB2, AGT, AGTR1, ALOX15, EDN1, GRK4, PTGS1, PTGS2, TLR4, VEGFA, and VEGFB.
EDN1 is a potent endogenous vasoconstrictor, has positive inotropic and chronotropic effects and mitogenic properties, influences homeostasis, and stimulates the renin-angiotensin-aldosterone and the sympathetic systems (1-3).
Pre-pro-EDN1 mRNA (2026 nucleotides) is the product of the human EDN1 gene (6836 nucleotides), which is located on chromosome 6p23-p24.
By sequencing the EDN1 gene of 56 unrelated Caucasians systematically, we identified four novel common genetic variations:
We therefore established standard PCR-restriction fragment length polymorphism (RFLP) methods for the 12 polymorphisms listed above and a melting peak analysis method using fluorescent probes (LightCycler assays) for the rapid detection of possibly functionally important EDN1 polymorphisms [except for +138/ex1ins/delA, for which a TaqMan assay already exists in our laboratory (11)].
org/content/vol50/issue2/) were chosen according to the EDN1 sequence (OMIM*131240; GenBank accession no.