restriction digest

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restriction digest

a unique family of DNA fragments produced by digestion of a given DNA molecule by a particular restriction enzyme; usually visualized by agarose gel electrophoresis and ethidium bromide or other fluorescent procedures.
References in periodicals archive ?
Another example is the NEBtools app, which houses the popular Double Digest Finder and Enzyme Finder, two online tools designed to assist researchers in setting up restriction enzyme reactions.
She would yell when I asked for another double digest.
To this effect, 340 clinical, veterinary, and environmental isolates from Argentina, Brazil, Chile, Colombia, Mexico, Peru, Venezuela, Guatemala, and Spain were typed by using M13 polymerase chain reaction-fingerprinting and orotidine monophosphate pyrophosphorylase (URA5) gene restriction fragment length polymorphsm analysis with Hhal and Sau961 in a double digest.
All 340 isolates were typed by PCR fingerprinting by using the minisatellite-specific oligonucleotide MI3 as a single primer and RFLP analysis of the URA5 gene with the restriction enzymes Sau96I and HhaI in a double digest.
Double digest system - increases the efficiency of alumina extraction from bauxite and improves the energy efficiency of the digestion process, relative to the type of single-digest system formerly used by Gramercy.
TM)] apps from NEB, which include the popular NEB Tools, Double Digest Finder and Enzyme Finder, as well as NEBuilder[sup.
With CutSmart Buffer, it is significantly easier to set up DNA digestion experiments, including double digest reactions.
1, 2011 /PRNewswire/ -- New England Biolabs (NEB), a world leader in the production and supply of reagents for the life science industry, has made its popular web tools, Double Digest Finder and Enzyme Finder, available as the "NEB Tools" app for Android(TM) phones and tablets.
The Double Digest Finder recommends optimum conditions for a double digest, including buffer, incubation temperature and supplement requirements.
Restriction sites of 10 restriction endonucleases were mapped in the two PCR-amplified mtDNA regions by using double digests.
To answer these questions, we conducted restriction site analyses on five individuals from each of 15 different Sebastes species common in Alaskan waters and mapped the sites using double digests to determine individual-based haplotypes.
Double digests were examined both in agarose and polyacrylamide by using 100- and 25-bp ladders.