Dot Blot

(redirected from Dot blotting)
A rapid—‘quick and dirty’—hybridization technique for semiquantifying a specific RNA and DNA fragment in a specimen without performing a more time-consuming Northern and Southern blot
References in periodicals archive ?
With regard to the regulation of survival pathway, using dot blotting, we found cardiac insulin-like growth factor-1 (IGF-1) and IGF-1 receptor mRNA levels to be significantly increased, indicating that compensative effects of IGF-1 survival signaling could occur.
To understand whether the effects of SHS on rat hearts are mediated through activating apoptotic pathways, including mitochondria-dependent and Fas death-receptor-dependent signalings, or through suppressing survival pathways, we examined the cardiac levels of signaling proteins and gene expression in these pathways by performing reverse-transcriptase polymerase chain reaction (RT-PCR), Western blotting, or dot blotting.
We used RNA dot blotting for the hybridization and detection of IGF-1 and IGF-1R mRNAs as described previously (Huang et al.
Except for the mRNA level of IGF-1R in the low-dose group, dot blotting analysis of cardiac survival factor showed mRNA of both IGF-1 and IGF-1R to have significantly greater increases than in the control group.
Dot blotting requires positive-control samples to monitor the sensitivity and specificity of each diagnostic blot.
The integrity of this product as positive-control DNA for the purposes of dot blotting was validated by probing a PCR product from a patient known to carry the A4136G base substitution in parallel dot blotting experiments.