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Dot Blot

   Also found in: Wikipedia 0.02 sec.
Dot Blot
A rapid—‘quick and dirty’—hybridization technique for semiquantifying a specific RNA and DNA fragment in a specimen without performing a more time-consuming Northern and Southern blot


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At the last stage of chromatography, a total of 22 fractions were collected and evaluated for their collagenase mRNA activities using dot blot analysis.
Western immunoblot and nondenaturing dot blot experiments were pursued by using brain, proventriculus, and adrenal homogenates of the 3 animals that were quantitated by real-time PCR, and 2 rabbit polyclonal antibodies raised against recombinant BDV strain V nucleoprotein (N) or P, as well as immune sera from BDV He/80--infected rats.
 
 
 
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