Trichloromethyl phythalimido disulfide was prepared by the reaction of potassium phthalimide with trichloromethane thiosulfenyl chloride as shown in Scheme 3.
The separated solid was filtered, washed with several portions of water, and air-dried to give trichloromethyl phthalimido disulfide (3.
These assays require the use of agents to reduce the disulfide
bond and liberate the thiol homocysteine.
Now, a team of scientists has demonstrated that tiny, round particles of tungsten disulfide
, typically 120 nanometers in diameter, make an even better lubricant than the powdered version.
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Loading of proteins with an excess of CysGly to provide an estimate of capacity for forming disulfides
with thiol-containing amino acids was performed in 125 mmol/L NaCl, 50 mmol/L Tris, pH 8.
Joseph Cecil Patrick (1892-1965), American scientist and pioneer in synthetic rubber, who discovered the synthesis of the polysulfide elastomer, the first non-hydrocarbon synthetic elastomer; who devised the first method for suspension polymerization, techniques for vulcanizing, and chemically plasticizing polysulfide polymers; and who, in the course of his research on cleavage and reformation of disulfide
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Reduced glutathione (GSH) is ubiquitous and abundant; it can be oxidized to its disulfide
form (GSSG) in response to an oxidative perturbation.
Glutathione and other thiols and disulfides
are then measured by spectrophotometry or by HPLC.
tHcy and tCys in EDTA plasma were analyzed simultaneously by HPLC after the reduction of plasma disulfides
with tris(2-carboxyethyl)phosphine, precipitation of proteins with trichloroacetic acid, derivatization with 7-fluoro-2,1,3-benzoxadiazole-4-sulfonate (SBD-F), and fluorescent detection as described previously (10) using cystamine as an internal standard.
Homocysteine (Hcy) is present in plasma primarily bound as disulfides
with itself, Cys, and albumin (~70%) (1-4).
In this study, rapid deproteinization preserved the disulfides
as free analytes for at least 7 days in storage at -20[degrees]C.