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On July 11, an 8-year-old unvaccinated boy developed a maculopapular rash diagnosed clinically as varicella and confirmed by direct flourescent antibody test on July 23.
5] Chlamydial cell culture technique has been refined and improved by the use of direct flourescent antibody confirmation, cycloheximide pretreatment of McCoy cells, and the use of shell vials and nontoxic collection materials.
A direct flourescent antibody test method was used to resolve discrepancies when the immunoassay was positive and the culture was negative.

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