GenBank: AF973617; Diplozoon aegyptensis, complete sequence.
GenBank: AF973618; Diplozoon guptai, complete sequence.
Diplozoon kashmirensis Kaw, 1950; Diplozoon aegyptensis Fischthal et Kuntz, 1963 and Diplozoon guptai  which were recovered during the present study are used for molecular study for the first time.
The sequence obtained from three different Diplozoon species were submitted to GenBank and their accession number acquired were AF973616; AF973617 and AF973618 (Table 4).
Pairwise alignments of Diplozoon species were made by using different softwares such as Gene Runner, DNA Dynamo, Chromas Pro.
Above Table shows that Diplozoon kashmirensis having GenBank accession number AF973616 mostly resembles with Diplozoon bliccae with an accession number AF369761.
From the Table 6 it is clear that Diplozoon aegyptensis having GenBank accession number AF973617 shows 94.
The present observation shows that Diplozoon guptai having GenBank accession number AF973618 shows 86.
During the present study it was observed that the alignment of nucleotide sequences with those of other Diplozoon species of D.
The length of the PCR product could be useful to distinguish Diplozoids from the genus Eu Diplozoon and Para Diplozoon from other Diplozoids [2,6].
a) Competition hypothesis: the installation of a first Diplozoon species prevents any other species from settling on the same gill.
Jiang NCH, Wu BH, Wang SX (1985) Four new species of parasitic Diplozoon from freshwater fishes of the subfamily Gobioninae.