dideoxynucleotide

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dideoxynucleotide

a synthetic molecule used in enzymatic DNA sequencing.
References in periodicals archive ?
A system for rapid DNA sequencing with fluorescent chain-terminating dideoxynucleotides.
PCR reactions, in the presence of the different dideoxynucleotides, are performed as described above.
Dye-tagged dideoxynucleotides (ddUTP, ddGTP, ddCTP, and ddATP) were added to terminate elongation (Sambrook & Russell 2001).
Sanger Sequencing, also called Sanger Dideoxy Sequencing, is the controlled interruption of enzymatic DNA replication through the use of dideoxynucleotides in primer-directed DNA extension to produce discrete DNA fragments (11,41) (Fig.
Solid phase capturable dideoxynucleotides for multiplex genotyping using mass spectrometry.
Nucleotide sequences were determined by oligonucleotide-directed dideoxynucleotide chain-termination sequencing reactions utilizing 300 ng of template DNA, forward (M13F) and reverse (M-13R) primers, fluorescently-labeled dideoxynucleotides and AmpliTaq FS DNA polymerase in a cycling sequencing method.
Then a mixture of all four dideoxynucleotides, each coupled to a different fluorescent dye, are added and primer extension performed.
15 U of thermosequenase, and a mixture of dideoxynucleotides (ddATP, ddCTP, and ddTTP) and the deoxynucleotide dGTP (64 [micro]mol/L each).
The cost of manufacturing primers with 3' modified locked nucleic acids is high, however, and primer with added 3' dideoxynucleotides is not widely available commercially, so this expertise-requiring modification must be performed in the laboratory.
Primer-extension techniques use labeled or unlabeled dideoxynucleotides to terminate the amplification in a sequence-dependent manner (10).
In our case, we have demonstrated that the C3 linker is indeed an excellent choice for such a blocking agent, but alternatives may include other carbon spacers and 3'terminal dideoxynucleotides, although the latter would add substantially to synthesis costs.
Our limited data (not shown) indicated that the extension products were retained in the order of C < G < T < A, which represent the 3' dideoxynucleotides of the products.