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dideoxynucleotide

   Also found in: Wikipedia 0.02 sec.
dideoxynucleotide
a synthetic molecule used in enzymatic DNA sequencing.


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Sanger Sequencing, also called Sanger Dideoxy Sequencing, is the controlled interruption of enzymatic DNA replication through the use of dideoxynucleotides in primer-directed DNA extension to produce discrete DNA fragments (11,41) (Fig.
Each positive PCR product was subjected to DNA sequencing with fluorescent-labeled dideoxynucleotide technology (BigDye Terminator Cycle Sequencing Ready Reaction Kit; Applied Biosystems, Foster City, CA, USA).
Nucleotide sequences were determined by oligonucleotide-directed dideoxynucleotide chain-termination sequencing reactions utilizing 300 ng of template DNA, forward (M13F) and reverse (M-13R) primers, fluorescently-labeled dideoxynucleotides and AmpliTaq FS DNA polymerase in a cycling sequencing method.
 
 
 
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