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dideoxynucleotide

   Also found in: Wikipedia 0.10 sec.
dideoxynucleotide
a synthetic molecule used in enzymatic DNA sequencing.


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Each positive PCR product was subjected to DNA sequencing with fluorescent-labeled dideoxynucleotide technology (BigDye Terminator Cycle Sequencing Ready Reaction Kit; Applied Biosystems, Foster City, CA, USA).
Molecular evidence for Anaplasma phagocytophilum in Israel by Keysary, Avi; Massung, Robert F.; Inbar, Moshe; Wallach, Arian D.; Shanas, Uri; Mumcuoglu, Kosta Y.; / Emerging Infectious Diseases
Nucleotide sequences were determined by oligonucleotide-directed dideoxynucleotide chain-termination sequencing reactions utilizing 300 ng of template DNA, forward (M13F) and reverse (M-13R) primers, fluorescently-labeled dideoxynucleotides and AmpliTaq FS DNA polymerase in a cycling sequencing method.
Nuclear small-subunit ribosomal RNA gene-based characterization, ... by Frasca, Salvatore, Jr. / Journal of Shellfish Research
Other approaches to improving sequencing technology involve methods that are independent of the Sanger dideoxynucleotide chain termination reaction or of electrophoretic separation of the termination products.
Near-term technology development for genome sequencing by Environmental Health Perspectives
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