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Nucleotide |
Also found in: Acronyms, Encyclopedia, Wikipedia, Hutchinson | 0.07 sec. |
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nucleotide /nu·cleo·tide/ (noo´kle-o-tid?) one of the compounds into which nucleic acid is split by action of nuclease; nucleotides are composed of a base (purine or pyrimidine), a sugar (ribose or deoxyribose), and a phosphate group. cyclic nucleotides those in which the phosphate group bonds to two atoms of the sugar forming a ring, as in cyclic AMP and cyclic GMP, which act as intracellular second messengers.
Nucleotide Any of a group of organic molecules that link together to form the building blocks of DNA or RNA. Mentioned in: Myotonic Dystrophy nucleotide any of a group of compounds obtained by hydrolysis of nucleic acids, consisting of a purine or pyrimidine base linked to a sugar (ribose or deoxyribose), which in turn is esterified with phosphoric acid. See also nucleoside, deoxyribonucleic acid. cyclic n's those in which the phosphate group bonds to two atoms of the sugar forming a ring, as in cyclic AMP and cyclic GMP, which act as intracellular second messengers. nucleotide sequences see dna sequencing. single nucleotide polymorphisms (SNPs) single base pair changes that distinguish one individual from another of the same species. |
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Amplification was done in a 50-[micro]L reaction volume containing 10 [micro]L 5x buffer, 2 [micro]L deoxynucleotide triphosphate mix (10 mmol/L), 2 [micro]L enzyme mix (all from QIAGEN), 2 [micro]L RNase inhibitor (Promega, Mannheim, Germany), 1. The incubation with a mixture containing digoxigenin deoxynucleotide triphosphate, unlabeled deoxynucleotide triphosphate, and terminal transferase enzyme was developed in a humidified chamber at 37[degrees]C for 1 hr. A 25-[micro]L reaction was conducted in a LightCycler (Roche, Indianapolis, IN, USA) with 10 mmol/L of each deoxynucleotide triphosphate, 600 nmol/L of each primer, 200 nmol/L of probe, and 3 [micro]L of RNA. |
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