denaturation

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denaturation

 [de-na″chur-a´shun]
a change in the usual nature of a substance, as by the addition of methanol or acetone to alcohol to render it unfit for drinking, or the change in the physical properties of a substance, such as a protein or nucleic acid, caused by heat or certain chemicals that alter tertiary structure.
protein denaturation any nonproteolytic change in the chemistry, composition, or structure of a native protein that causes it to lose some or all of its unique or specific characteristics.

de·na·tur·a·tion

(dē-na'tyū-rā'shŭn),
The process of becoming denatured.

denaturation

/de·na·tur·a·tion/ (de-na″cher-a´shun) destruction of the usual nature of a substance, as by the addition of methanol or acetone to alcohol to render it unfit for drinking, or the change in the physical properties of a substance, as a protein or nucleic acid, caused by heat or certain chemicals that alter tertiary structure.

denaturation

[dēnā′chərā′shən]
Etymology: L, de + natura, natural
1 the alteration of the basic nature or structure of a substance.
2 the process of making a potential food or beverage substance unfit for human consumption although it may still be used for other purposes, such as a solvent.

de·na·tur·a·tion

(dē-nā'chŭr-ā'shŭn)
The process of becoming denatured.

denaturation

1. Alteration in the folding pattern of a protein by heat or chemical reaction from its physiological conformation to an inactive shape.
2. In the case of DNA or RNA the conversion from a double-stranded structure to a single stranded structure, usually by heating. This is an essential stage in the POLYMERASE CHAIN REACTION.

denaturation

the breakdown of the bonds forming the quaternary, tertiary and secondary structures of PROTEINS and NUCLEIC ACIDS, a process that can be caused by various agents, such as excess heat, strong acids or alkalis, organic solvents and ultrasonic vibration. In some cases, denaturation is irreversible as in, for example, the heating of egg albumen to give solid egg white. When denaturation occurs in enzymes, they are inactivated, with major effects in living organisms. Denatured nucleic acids can often be returned to their original configuration in the process of renaturation.

denaturation

1. a change in the usual nature of a substance, as by the addition of methanol or acetone to alcohol to render it unfit for drinking.
2. in proteins and nucleic acids produced by heat or certain chemicals, usually results in loss of function. In proteins, various noncovalent bonds are disrupted resulting in unfolding of the polypeptide chain; in nucleic acids hydrogen bonds between nucleotides are disrupted converting double-stranded molecules or parts of them into single-stranded forms.

protein denaturation
any nonproteolytic change in the chemistry, composition or structure of a native protein which causes it to lose some or all of its unique or specific characteristics.
References in periodicals archive ?
The amplification was performed with 1 min denaturating at 95[degrees]C followed by 35 cycles of 94, 52 and 72[degrees]C, 1 min each with a 2.
The PCR cycle program consisted of an initial denaturating cycle of 95[degrees]C for 10min and 40 repetitions of cycles of 95[degrees]C for 10s and 60[degrees]C for 65s [15].
Heteroduplex formation was induced by denaturating PCR products at 95[degrees]C for 5 minutes followed by gradual re-annealing from 95[degrees]C to 23[degrees]C (room temperature) prior to analysis.
Cytoskeletal proteins were separated by 10% SDS-polyacrylamide gel electrophoresis, under denaturating conditions, and then transferred to Immobilion-P (Millipore, Bedford, MA, USA) membranes.
The starch was modified during tortilla cooking up to the denaturating temperature.
1992), soybean cultivars (Buehler, 1989), are not suitable for peas because pea globulins would dissociate into subunits under RP-HPLC denaturating conditions generally used in these studies.
The protein of interest was both in the insoluble and the soluble fraction; therefore, purification was performed under denaturating and native conditions using affinity chromatography.
In addition, we tested the application of denaturating high-performance liquid chromatography (DHPLC) for the identification of the ITS PCR product to distinguish M.
Effects and agents driving and facilitating the thiol/disulfide exchange reaction are higher temperature, denaturating agents like SDS, chaotropic ions, and basic conditions.