primer

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prim·er

(prī'mĕr),
1. A molecule, which may be a small polymer, that initiates the synthesis of a larger structure. Synonym(s): starter
2. A pheromone that causes a long-term physiologic change.

primer

(prī′mər)
n.
Genetics A segment of DNA or RNA that is complementary to a given DNA sequence and that is needed to initiate replication by DNA polymerase.

primer

[prī′mər]
1 a short piece of DNA or RNA complementary to a given DNA sequence that acts as a point at which replication can proceed, as in a polymerase chain reaction.
2 a molecule, such as a small polymer, that induces the synthesis of a larger molecule.

primer

1. A short RNA sequence paired with one DNA strand that provides a free 3’-OH end on which synthesis of a deoxyribonycleotide chain can start.
2. The short nucleotide sequence used to start the POLYMERASE CHAIN REACTION (PCR) process.

primer

a short OLIGONUCLEOTIDE, which is required for the functioning of various POLYMERASES. In DNA REPLICATION, the primer is a short RNA sequence that anneals to the DNA TEMPLATE and is extended by DNA POLYMERASE. In the POLYMERASE CHAIN REACTION, the primer is a short oligonucleotide that initiates the PCR. In OLIGONUCLEOTIDE-DIRECTED MUTAGENESIS, the synthetic oligonucleotide used to introduce the mutation is a mutagenic primer that initiates DNA synthesis.

primer

oligonucleotide which is hydrogen-bonded to the template strand of DNA; required for the replication of DNA by polymerase.

random primer method
a method of labeling DNA in vitro.
primer walking
an orderly method for sequencing DNA which involves obtaining some sequence, then making an oligonucleotide primer to the end of the sequence obtained and using it as a primer for the next sequencing reaction and repeating this until the whole sequence is determined.
References in periodicals archive ?
In this project, the degenerate primers, PALFor1EcoR1 (5'GAAGATCTTCGVTTCTTGAATGABGGAATYTTTYGGNA-3') and PALRevBgIII (5'- CGGAATTCCGTTADCAHATNGGAAGAGGAGCACCA-3') successfully amplified the corresponding PAL promoter gene sequence in orchid (Dendrobium crumenatum).
striatellus was amplified with the degenerate primers (Table 1) and the specific primers (Table 2).
To analyze the possible presence of other porcine lymphotropic herpesviruses in samples that reacted positively for OvHV-2, a nested PCR with degenerate primers (6,7) was applied.
These consensus degenerate primers are effective for detecting many herpesviruses from vertebrate hosts.
Because the degenerate primers used were designed to cover regions that are generally highly conserved, regions of the genome that are typically more variable in other pneumoviruses may show greater differences between the canine pneumovirus and MPV.
For molecular typing, we used repetitive-element PCR with 18-mer degenerate primers REP1R-Dt (3'-CGGNCTACNGCNGCNIII-5') and REP2Dt (3'-CATCCGGNCTATTCNGCN-5') (N = A, C, G, and T; I = inosine) (11).
A second set of panspecific 16S rDNA degenerate primers (5'-GTTTGATYCTGGCTYAGARCKAACG-3' and 5'-CCSSTACGGMTACCTTGTTACG-3') confirmed the presence of Brachyspira spp.
Genomic DNA was prepared from the 105 isolates, and a PCR with degenerate primers capable of detecting all CTX-M enzymes identified a single positive S.
Previous demonstration that degenerate primers could be used in LAMP assays (6) led us to use the same approach for designing a set of degenerate primers for the targeted region (online Appendix Figure 2B).