Different studies suggest that transcription-associated recombination (TAR) is in large part due to collisions between transcription and replication, but increasing evidence indicate that R-loops, formed by a DNA-RNA hybrid
and a displaced single-stranded DNA, may be a major determinant of genome instability.
Such a DNA-RNA hybrid
would be resistant to both RNase and DNase activity.
The added sequence will bind up the messenger RNA with the same powerful bonding that holds together a DNA double helix, thus creating a freak double-stranded RNA or a DNA-RNA hybrid
The H stands for hybrid, and this enzymatic activity degrades away the RNA pair of annealed DNA-RNA hybrids
In each case, specific HPV DNA-RNA hybrids
Specificity, however, may be further improved because biotinylated oligonucleotides specific for HPV DNA sequences are used to capture HPV DNA-RNA hybrids
(instead of anti-DNA-RNA polyclonal antibody used previously) onto streptavidine-coated wells of a microtiter plate.