DNA-DNA hybridization


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DNA-DNA hybridization

the formation of double-helical DNA from two complementary single strands. It is used to compare genome relationships between different species.
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In 1995, a comprehensive study on lipophilic corynebacteria demonstrated by DNA-DNA hybridization the similarity between a reference strain of C.
Characterization of Vibrio metschnikovii and Vibrio gazogenes by DNA-DNA hybridization and phenotype.
Phenotypic, protein profile, 16S rDNA sequence, and DNA-DNA hybridization analyses identified the human strain as H.
heilmannii" isolate, Rigshospitalet 53 (R-53), and other Helicobacter strains for comparison were cultured by using conditions described earlier for phenotypic (12), whole-cell protein (13), 16S rRNA sequence (12), and DNA-DNA hybridization (10) analyses.
DNA-DNA hybridization studies of clinical isolates CV004, CV005, CV006, and CV015 with labeled patient isolate CV002 showed RBRs of 66 to 96% and %D values of 0.
DNA-DNA hybridization analysis also supports the novel classification with its low relative binding ratios and higher percent divergence from all other M.
The metric properties of DNA-DNA hybridization dissimilarity measures.
Statistical issues arising in the analysis of DNA-DNA hybridization data.
Conventional electrical SNP identification technologies, which use DNA-DNA hybridization reactions to identify the differences in DNA sequences, are not only inaccurate, but also require specialized and expensive electrodes to which artificial DNA needs to be attached.
One [micro]g of genomic DNA from numerous H parainfluenzae or H influenzae strains was spotted on a nylon membrane and the presence of tonB sequences was determined through DNA-DNA hybridization with a digoxigenen-labeled probe (DIG DNA Labeling and Detection Kit; Roche, Indianapolis, IN) produced from a tonB-specific PCR product obtained using H parainfluenzae 4201 genomic DNA as the template.
Covalently immobilized DNA plate for luminometric DNA-DNA hybridization to identify viridans streptococci in under 2 h.
32]P-labeled cjp5 DNA probe was used immediately in DNA-DNA hybridizations to screen isolated C.