Draft genome sequences of strains representing each of the Elizabethkingia genomospecies previously determined by DNA-DNA hybridization
Additionally, the data on DNA-DNA hybridization
was required with all the validly recognized species having more than 97% similarity of 16S rRNA gene sequence.
is considered the gold standard of oral biofilm analysis.
The sample was divided in two portions, one designated for the BANA Test and the other one for the Checkerboard DNA-DNA hybridization
Conventional electrical SNP identification technologies, which use DNA-DNA hybridization
reactions to identify the differences in DNA sequences, are not only inaccurate, but also require specialized and expensive electrodes to which artificial DNA needs to be attached.
One [micro]g of genomic DNA from numerous H parainfluenzae or H influenzae strains was spotted on a nylon membrane and the presence of tonB sequences was determined through DNA-DNA hybridization
with a digoxigenen-labeled probe (DIG DNA Labeling and Detection Kit; Roche, Indianapolis, IN) produced from a tonB-specific PCR product obtained using H parainfluenzae 4201 genomic DNA as the template.
Bacterial species determination from DNA-DNA hybridization
by using genome fragments and DNA microarrays.
Covalently immobilized DNA plate for luminometric DNA-DNA hybridization
to identify viridans streptococci in under 2 h.
In this study, I use a tree based on DNA-DNA hybridization
distances as an independent estimate of phylogeny.
chelonae-abscessus group, we performed a comprehensive analysis of the potentially new species, including DNA-DNA hybridization
, multilocus sequencing, and deep multilocus sequencing.
Counts of 38 bacterial species were determined in each sample, using the Checkerboard DNA-DNA hybridization
technique1 as previously described by Faveri et al.
between the pseudoobscura and affinis subgroup species yielded a mean [Delta]Tm of 2.