DNA polymerases


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DNA polymerases

Enzymes that bring about the synthesis of a daughter strand of DNA on the basis of a complementary DNA template. They are involved in DNA replication and repair, and act by adding deoxynucleotide triphosphates to the 3’-OH group of the new DNA strand. These enzymes not only synthesize new DNA but proof-read the new strand and remove incorrect nucleotides and replace them with the correct ones.
References in periodicals archive ?
Single-molecule studies of the effect of template tension on T7 DNA polymerase activity.
This anneals to the single-stranded DNA product of the previous step, providing a starting point for another of M-MLV RT's activities: a classical DNA polymerase function.
Another tenable explanation for the presence of multiple specialised DNA polymerases in vertebrates is that each evolved to accurately bypass a particular type of naturally occurring base damage.
However, results from such amplifications can be compromised by a DNA polymerase incapable of evenly amplifying diverse types of sequences.
Cloning and sequencing of lcr1 by Pfu DNA polymerase: To determine whether the sequence variants were created during PCR due to Taq polymerase error, lcr1 was amplified from the parasite genomic DNA by Pfu DNA polymerase (Fermentas Co.
The template DNA of unknown sequence is combined in a buffered solution with the four dNTPs (dATP, dCTP, dGTP, dTTP), the four ddNTPs (ddATP, ddCTP, ddGTP, ddTTP), primers and a DNA polymerase (Fig.
Investigators have for many years created crystals of DNA polymerases and shone X rays through them to reveal the precise locations of the molecules' many atoms.
DNA polymerases require a pre-existing template--a single strand of DNA which is used to direct formation of its complement.
Lucigen has offered its popular EconoTaq DNA Polymerase and EconoTaq PLUS 2X Master Mixes for several years, garnering a reputation for robustness and cost effectiveness.
We used 10 DNA samples to compare the performance of 3 commercially available DNA polymerases in ASPE, including Platinum[R] GenoType Tsp DNA Polymerase, TITANIUM Taq DNA Polymerase, and TaKaRa Taq HS DNA Polymerase.
Moreover, the 1953 publication of Watson and Crick launched exquisitely detailed characterization of how DNA is faithfully replicated, and from this came an understanding of the role that DNA polymerases and such processes as recombination must play in the generation of DNA sequence changes.
These engineered DNA polymerases contain unique amino acid modifications that confer dramatic improvements to the functionality of the enzymes when compared to wild-type polymerases.