DNA polymerases


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DNA polymerases

Enzymes that bring about the synthesis of a daughter strand of DNA on the basis of a complementary DNA template. They are involved in DNA replication and repair, and act by adding deoxynucleotide triphosphates to the 3’-OH group of the new DNA strand. These enzymes not only synthesize new DNA but proof-read the new strand and remove incorrect nucleotides and replace them with the correct ones.
References in periodicals archive ?
In this study, we verified the possibility of incorporating a number of modified nucleotides by certain types of DNA polymerases.
Archaeal dUTPase enhances PCR amplifications with archaeal DNA polymerases by preventing dUTP incorporation.
However, researchers have been trying to produce such enzyme from various versions of Taq DNA polymerase gene via genetic engineering.
Another tenable explanation for the presence of multiple specialised DNA polymerases in vertebrates is that each evolved to accurately bypass a particular type of naturally occurring base damage.
We tested this unique substrate out of curiosity,34) and thereby found that DNA polymerases sensitively recognize dDsT[P.
Cloning and sequencing of lcr1 by Pfu DNA polymerase: To determine whether the sequence variants were created during PCR due to Taq polymerase error, lcr1 was amplified from the parasite genomic DNA by Pfu DNA polymerase (Fermentas Co.
The template DNA of unknown sequence is combined in a buffered solution with the four dNTPs (dATP, dCTP, dGTP, dTTP), the four ddNTPs (ddATP, ddCTP, ddGTP, ddTTP), primers and a DNA polymerase (Fig.
coli cells begin to starve, the bacteria quadruple their expression of DNA Polymerase IV (Pol IV), a mutation-causing enzyme that is notoriously bad at copying DNA accurately.
Overseeing this process is a unique enzyme known as DNA polymerase, that "rides" on board the existing strand much like a train on a single track, reading its genetic sequence to form a matching strand.
Investigators have for many years created crystals of DNA polymerases and shone X rays through them to reveal the precise locations of the molecules' many atoms.
Effective removal of unincorporated nucleotides ologonukleotidi, mineral oils and thermostable DNA polymerases.
Several methods have been developed to measure the activity of DNA polymerases, but complexity, time requirements, and specialized instrumentation have prevented their widespread use.