In this study, we verified the possibility of incorporating a number of modified nucleotides by certain types of DNA polymerases
Archaeal dUTPase enhances PCR amplifications with archaeal DNA polymerases
by preventing dUTP incorporation.
However, researchers have been trying to produce such enzyme from various versions of Taq DNA polymerase
gene via genetic engineering.
Another tenable explanation for the presence of multiple specialised DNA polymerases
in vertebrates is that each evolved to accurately bypass a particular type of naturally occurring base damage.
We tested this unique substrate out of curiosity,34) and thereby found that DNA polymerases
sensitively recognize dDsT[P.
Cloning and sequencing of lcr1 by Pfu DNA polymerase
: To determine whether the sequence variants were created during PCR due to Taq polymerase error, lcr1 was amplified from the parasite genomic DNA by Pfu DNA polymerase
The template DNA of unknown sequence is combined in a buffered solution with the four dNTPs (dATP, dCTP, dGTP, dTTP), the four ddNTPs (ddATP, ddCTP, ddGTP, ddTTP), primers and a DNA polymerase
coli cells begin to starve, the bacteria quadruple their expression of DNA Polymerase
IV (Pol IV), a mutation-causing enzyme that is notoriously bad at copying DNA accurately.
Overseeing this process is a unique enzyme known as DNA polymerase
, that "rides" on board the existing strand much like a train on a single track, reading its genetic sequence to form a matching strand.
Investigators have for many years created crystals of DNA polymerases
and shone X rays through them to reveal the precise locations of the molecules' many atoms.
Effective removal of unincorporated nucleotides ologonukleotidi, mineral oils and thermostable DNA polymerases
Several methods have been developed to measure the activity of DNA polymerases
, but complexity, time requirements, and specialized instrumentation have prevented their widespread use.