DNA ligases

DNA ligases

Enzymes that reconstitutes the double strand in a DNA molecule that has a discontinuity in one strand. DNA ligases join up the broken strand by catalyzing a PHOSPHODIESTER BOND at this point. From the Latin ligare , to bind.
References in periodicals archive ?
Restriction enzymes and T4 DNA ligases were purchased from New England Biolabs (Ipswich, MA).
Each ligation reaction (20 [micro]L) contained the restriction digestion product (10 [micro]L), 1 x T4 DNA ligase buffer with EDTA, 0.
DNA ligases then join the DNA molecules with "sticky ends" to bacterial plasmids that also have been cleaved by restriction enzymes.
After trying T4 DNA ligases from different vendors, we decided to use highly concentrated enzyme (10 Weiss units/[micro]L) (23) provided by Epicentre.
For the annealing step, 500 pmol of appropriate 24mer and 20mer oligonucleotides were mixed in a 10-[micro]L volume of 1 x T4 DNA ligase buffer [33 mmol/L Tris-acetate (pH 7.
L of reaction mixture containing 1 x T4 DNA ligase buffer supplemented with 1 mmol/L ATP, 60 ng of partially digested DNA,10 U of highly concentrated T4 DNA ligase (Epicentre), and 100 fmol of the appropriate Indexer (3-fold molar excess over 5' overhangs).
Assuming that the 3'-end of the first primer matches perfectly with the target DNA, both primers can be ligated by DNA ligases (e.
Earlier studies had revealed that bacteriamutants with a defective DNA ligase also show characteristic chromosomal and DNA problems.
Results suggest it is the DNA ligase Ithat is defective.