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DNA ligase |
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DNA ligase /DNA li·gase/ (li´gas) a ligase that catalyzes the linkage between two free ends of double-stranded DNA chains by forming a phosphodiester bond between them, as in the repair of damaged DNA. |
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? Mentioned in | ? References in periodicals archive | |
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Five microliters of DNA digest was used in a ligation reaction containing 130 [micro]g/mL Bg/II adaptor-oligonucleotide and 13 [micro]g/mL Csp6I adaptor-oligonucleotide (Invitrogen) (14), 10x T4 DNA ligase buffer, T4 DNA ligase (1 U/[micro]L) (Amersham Pharmacia), and TAC-buffer in a final volume of 20 [micro]L. Inhibition of DNA ligase activity by arsenite: a possible mechanism of its comutagenesis. Earlier studies had revealed that bacteriamutants with a defective DNA ligase also show characteristic chromosomal and DNA problems. |
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DNA ligase
