Formation of the nucleosomal DNA ladder
is the hallmark of apoptosis so analysis of the pattern of DNA isolated from the cells exposed to different stress inducers was done.
Gels were normalized using the DNA ladder
markers as reference.
Digests were subjected to gel electrophoresis using the Chef Mapper System (Bio-Rad Laboratories, Hercules, CA, USA) with a bacteriophage X DNA ladder
Their sizes were estimated by comparison with a commercial 1-kb plus DNA ladder
A 100 bp DNA ladder
(Takara Mirus Bio, Madison, Wisconsin) was used as a size marker.
Sally Williams, owner of Lilly, a Jack Russell cross, puts her canine knowledge to the test IF your dog's soulful eyes or sneaky behaviour seem almost humanlike at times, there's good reason - you share about 650 million base pairs, or rungs, on the DNA ladder
Using these pieces, they construct their own twisting model of the DNA ladder
, with careful attention to base pairing.
Using a microfluidic device, researchers at NIST have demonstrated a separation of a DNA ladder
with fragments ranging from 50 to 550 base pairs in 4 min.
(MBI Fermentas) 250 ug , RNA easy mini kit Qiagen Each kit of 50 Rxns , DNA easy blood and Tissue kit Qiagen Each kit of 50 Rxns , Quaigen one step RT-PCR kit Each kit of minimum 20 Rxns ,Rainbow marker for SDS PAGE Biorad 2X250 ul ,Magic marker kit for SDS PAGE Invitrogrn Each vial for minimum 20 Rxns , MOPS (Molecular Biology Grade) Sigma 250ml , Protease inhibitor tablets (Molecular Biology Grade) Sigma --Biotechnology & molecular medicine
(5 [micro]L; Phenix Research Products 100-bp DNA ladder
) is loaded in the first well; in the subsequent wells, 20 [micro]L of the PCR and loading dye mixtures are loaded.
A DNA ladder
was also used to allow quantification of the weights of the different bands of DNA separated by the electrophoresis.
The limit of detection here is ~10 ng of DNA (as calculated by the DNA ladder