DNA helicase


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DNA helicase

an enzyme that catalyzes the energy-dependent unwinding of the DNA double helix during DNA replication.

BLM

A gene on chromosome 15q26.1 that encodes a DExH box-containing DNA helicase with both DNA-stimulated ATPase and ATP-dependent DNA helicase activities, which is thought to suppress inappropriate recombination events.
 
Molecular pathology
BLM mutations cause Bloom syndrome.
References in periodicals archive ?
Instead, in vivo DNA replication methods for most organisms rely on a class of enzymes known as DNA helicases, which act to locally unwind dsDNA to create small ssDNA regions suitable for polymerase activity.
Disease variants of the human mitochondrial DNA helicase encoded by C10orf2 differentially alter protein stability, nucleotide hydrolysis and helicase activity.
Mutations in any one of these genes, such as in the DNA helicase gene associated with Werner's syndrome, may trigger major portions of the aging phenotype.
These patients are homozygous for the BLM gene on the long arm of chromosome 15, which has also been recently shown to encode a DNA helicase [49].
In both cases, fluoroquinolones appear to trap the enzyme on DNA during the topoisomerization reaction, forming a physical barrier to the movement of the replication fork (4), RNA polymerase (5), and DNA helicase (6).
It is caused by null mutations at WRN locus, which codes for a member of RecQ family of DNA helicases.
Other topics include mediation and modulation of membrane-cytoskeleton interactions, metal binding affinity and selectivity in metalloproteins, translocation and unwinding mechanisms of RNA and DNA helicases, and the structure of eukaryotic RNA polymerases.
The assay can be used for any enzyme class that produces ADP, including protein, lipid or carbohydrate kinases, ATPases, DNA helicases, and carboxyltransferases.