restriction digest

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restriction digest

a unique family of DNA fragments produced by digestion of a given DNA molecule by a particular restriction enzyme; usually visualized by agarose gel electrophoresis and ethidium bromide or other fluorescent procedures.
References in periodicals archive ?
Apoptosis was evaluated by fluorescent microscopy, transmission electron microscopy, flow cytometric analysis of annexin V-FITC and propidium iodide-stained cells and DNA fragmentation.
The Covaris AFA process is extensively cited in peer-reviewed research articles on DNA fragmentation, ChIP, FFPE DNA extraction, metabolomics, small molecule extraction, proteomics, and nanoformulation.
Tenders are invited for Purchase of DNA fragmentation equipment.
2012) reported no significant associations of plasma and semen PFOS and PFOA concentrations with reproductive hormones or select semen quality end points among 256 men attending infertility clinics; these authors did not consider either morphology or DNA fragmentation.
We can test men for DNA fragmentation of the sperm, which increases with age and impacts fertilisation and continuation of a healthy pregnancy.
Additionally, genetic factors have been associated with predisposition to sperm DNA fragmentation in infertile men.
2010) reported that oleanolic acid inhibited the proliferation of liver cancer cells by inducing apoptosis after increasing DNA fragmentation levels and decreasing the mitochondrial membrane potential.
All of MTT assay, apoptotic bodies formation assay and DNA fragmentation assay had three repeated and was performed with Student's t-test for statistical analysis.
DNA Fragmentation Index Calculation: Extent of DNA denaturation (damage) was expressed in terms of DFI, which is the ratio of red to total (red plus green) fluorescence intensity, i.
To mention a few: male age, paternal exposure to toxic material, occupation, (27-29) anomalies of chromatin organization, reduced decondensation of chromatin, DNA strand breaks DNA fragmentation (30-36) and elevated levels of sperm aneuploidy.
The objective of the present study was to evaluate whether internucleosomal DNA fragmentation, a biochemical characteristic of apoptosis, could be detected in chronically hypoperfused myocardium containing apoptotic myocytes.