DNA footprinting


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A technique in which a DNA molecule is ‘incubated’ with a protein that binds to a specific site along the double helix; the DNA-binding protein complex is then subjected to restriction endonuclease digestion, which reduces the entire DNA to mono- and oligonucleotide fragments, except for the portion of the DNA molecule that was ‘protected’ from digestion by the binding protein; removal of the protein by simple chemical means—e.g., by gel electrophoresis—allows the study of DNA and binding protein interaction

DNA footprinting

see FOOTPRINTING.
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Their topics include the serial analysis of gene expression as a tool to analyze the cardiac transcriptome, in situ hybridization to study the localization of cardiac gene expression, mapping transcriptional start sites and in silico DNA footprinting, and lintivirus-mediated gene expression.
Conventional methods for detecting DNA-binding proteins are electrophoretic mobility shift assay (EMSA) [3] (7), DNA footprinting (8), ELISA (9), and Southwestern blotting (10).
Unlike DNA fingerprinting, which can highlight tiny discrepancies between the genetic material of two individuals, DNA footprinting reveals where proteins stick to DNA.