DNA amplification


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amplification

 [am″plĭ-fĭ-ka´shun]
the process of making larger, such as the increase of an auditory or visual stimulus, as a means of improving its perception.
DNA amplification artificial increase in the number of copies of a particular DNA fragment into millions of copies through replication of the segment into which it has been cloned, a type of nucleic acid amplification.
gene amplification a process by which the number of copies of a gene is increased in certain cells because extra copies of DNA are made in response to certain signals of cell development or of stress from the environment. In humans this process is seen most often in malignant cells.
nucleic acid amplification amplification of a specific nucleic acid sequence, such as to test for presence of a given virus or bacteria in a sample. Types include DNA amplification, ligase chain reaction, and polymerase chain reaction.

DNA amplification

artificial increase in the number of copies of a particular DNA fragment into millions of copies through replication of the segment into which it has been cloned, a type of nucleic acid amplification.

DNA amplification

Molecular diagnostics Any method used to ↑ the copy number of a sequence of DNA. See Cycling probe technology, Gap LCR–gap ligase chain reaction, Gene amplification, NASBA–nucleic acid sequence-based amplification, PCR, SDA–strand-displacement amplification, TMA–transcription-mediated amplification.
References in periodicals archive ?
However, despite all of these precautions, the polymerase chain reactions (PCRs) failed to achieve satisfactory amplification, omitting DNA amplifications of 100- to 200-bp fragments, which were exactly those we were looking for.
The origin of bermudagrass (Cynodon) off-types inferred by DNA amplification fingerprinting.
The newer DNA amplification methods are also far more sensitive in terms of the number of organisms required for a positive test compared with the first culture-independent test, the enzyme-linked immunosorbent assay (ELISA).
Kinetic PCR analysis: real-time monitoring of DNA amplification reactions.
Despite variation in qRT-PCR, endpoint analysis by gel electrophoresis yielded no complete inhibition of Xylella DNA amplification by either orange oil or Stickem[R] (Fig.
The new device can identify DNA amplification products in a real-time manner and rapidly conduct quantitative analysis.
DNA amplification is a powerful technique that has had an immense impact on scientific research in the past 2 decades.
Chambers for on-chip DNA amplification, cycle sequencing reactions and sample clean-up have been also developed, and experiments to integrate these steps, an approach that effectively places much of the actual process and process control onto the device, are being conducted in several laboratories.
These systems allow for fluorescence monitoring during the DNA amplification process.
A powerful technique termed DNA amplification fingerprinting (DAF) has been used successfully to distinguish variations within species of centipedegrass [Eremochloa ophiuroides (Munro) Hack] (Weaver et al.
Beckman, president and CEO of Intergen said, "This is a very exciting technology platform for DNA amplification and detection that provides sensitive, quantitative results without the need for further processing after amplification.
The LadaTech patent covers a process of DNA amplification required for the Sample Preparation and Cluster Station amplification steps of the Illumina DNA sequencing systems.