DAPI


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DAPI

Abbreviation for 4'6-diamidino-2-phenylindole·2HCl. See: DAPI stain.
References in periodicals archive ?
The larva was then placed on a glass depression slide into an approximately 100-pL mixture of DAPI and Syto RNASelect stains.
We stained gingival cells grown on cover slips with DAPI to visualize the nuclei.
The DAPI is a C-based DLL that can be used by all programming languages that support Unicode.
Meanwhile, morphologic changes in the nucleus and cytoskeleton of the treated cells were observed under LCSM with DAPI and tubulin Alexa Fluor 488 staining; whereas, the mitochondrial membrane potential was detected with Mito Tracker Red CMXRos staining.
DAPI staining these chromosome regions showed negative markings which reveal richness in GC base pairs (Figs.
Next, the cells were treated with FITC-labeled goat anti-rabbit IgG (1:100), incubated at 37[degrees]C in a wet box for 30 min, and stained in DAPI for 1-2 min.
6) Protein levels of VIM mesenchymal cell marker shown by DAPI and VIM immunofluorescence (bar = 20 [micro]m).
5 [micro]m) images were captured for each histospot in each of the DAPI, AlexaFluor-546, and Cy5 fluorescent channels by using a modified, computer-controlled epifluorescence microscope (Olympus BX-51 with xy stage and z controller, Olympus America Inc, Center Valley, Pennsylvania) illuminated by a high-pressure mercury bulb (Photonics Solutions, Edinburgh, United Kingdom) coupled with a high-resolution monochromatic camera (PCO-Tech, Romulus, Michigan).
Later it is stained with DAPI or Diff-Quik reagent, and finally observed by fluorescence or light microscope (26, 27).