Phosphorylation-dependent ubiquitination of cyclin
D1 by the SCF(FBX4-alphaB crystallin) complex.
We didn't expect cyclin
D3 to ramp up beta cell replication so strongly when it was used with either cdk4 or cdk6.
-dependent kinase inhibitors in normal regulation of proliferation and differentiation.
The rise and fall of cyclin
concentrations move a cell through the four stages of its life cycle: growth, DNA replication, preparation for cell division, and cell division itself (SN: 4/23/94, p.
Antibodies against p27 (GTX100446), Cyclin
D1 (GTX27958) and Cyclin
E (GTX27959) were obtained from Gene Tex (Irvine.
Nikaido et al (11) reported that about 40% of endometrial carcinomas overexpress cyclin
D1 and proposed that cyclin
D1 dysregulation may have a role in endometrial carcinogenesis.
The journal article is entitled "G(1) arrest of U937 cells by ONCONASE is associated with suppression of cyclin
D3 expression, induction of p16(INK4A), p21(WAF1/CIP1) and p27(KIP) and decreased pRb phosphorylation".
Rabbit monoclonal antibody against activated caspase-3, PARP (cleaved p85), cyclin
-dependent kinase 1 (Cdk1), phospho-Cdkl (Tyr15), cell division cycle (Cdc) 25c, and GAPDH were obtained from Santa Cruz (Santa Cruz, CA, USA).
D1 gene amplification was observed diffusely in both components, whereas no CD1 gene amplification was observed in the adjacent nonneoplastic tissue.
Effect of Extract on Bcl-2, Cyclin
B1 and Ki67 Expression
The main components of RB pathway involves five family proteins; retinoblastoma tumor suppressor RB- family of pocket proteins (p110, p107, p130), E2F-family of transcription factors (heterodimers of E2F1-8 with DP1-2), cyclins
(D and E-type), cyclin
-dependent protein kinases (cdk4, cdk6) and cyclin
-dependent kinase inhibitor CDKIs (p16Ink4a, p15Ink4b, p18Ink4c and p19Ink4d).
To investigate whether CA blocked the progression through the cell cycle by inhibiting the levels of the Cyclins
and consequently the activities of CDKs, we first performed immunoblot analysis of CA treated and control PT2 cells with specific antibodies directed against Cyclin
A and B1.