Sperm cryopreservation of tiete tetra Brycon insignis (Characiformes): effects of cryoprotectants
, extenders, thawing temperatures and activating agents on motility features.
Individual samples were randomly assigned different concentrations and type of cryoprotectant
5-mL French straws, and frozen at a cooling rate of 20-25[degrees]C/min (sample probe temperature) from 5[degrees]C to -80[degrees]C within 20 min after mixing with cryoprotectant
In order to decrease the damage of osmotic and toxic stress, cryoprotectant
must be added in a stepwise manner.
Osmotic tolerance limits and effects of cryoprotectants
on motility of bovine spermatozoa.
This decrease is more pronounced for the powder without protecting compounds, which leads us to say that cryoprotectants
slowed the oxidation phenomenon during freeze-drying and during 60 days of storage.
Post-thaw spermatozoa plasma membrane integrity and motility of stallion semen frozen with different cryoprotectants
Vitrification by ultra-fast cooling at a low concentration of cryoprotectants
in a quartz micro-capillary: a study using murine embryonic stem cells.
This differs from freezing in that 60 percent of the water inside the cells is replaced with protective chemicals called cryoprotectants
before the temperature is dropped.
Although initial survival, fertilisation and cleavage rates for cryopreserved oocytes were relatively poor, the advancements made in cryoprotectants
and method protocols in the recent past have caused these rates to improve steadily.
In cryopreservation, cryoprotectants
such as sucrose, DMSO, Ethylene glycol, glycerol are used to protect the plant material from damaging effects due to freezing.
Upon removal of the cryoprotectants
, the embryos were incubated for 72 hours and assessed for blastocyst development based on morphology.