15] Anti-dsDNA antibodies were measured by the Crithidia luciliae
indirect immunofluorescence test,  which is regarded as a reference method because of high specificity.
Anti-dsDNA was measured semiquantitatively using Fluoro nDNA test (MBL, Japan) that employed Crithidia luciliae
indirect immunofluorescent test (CLIFT) method.
Como controles positivos y para probar la condicion del anticuerpo secundario se anadieron anticuerpos secundarios marcados con isotiocianato de fluoresceina (FITC) a cortes de tejido renal de rata y a laminas de Crithidia luciliae
(Kallestad, USA), que habian sido previamente enfrentados al suero de un paciente con diagnostico establecido de lupus eritematoso sistemico, positivo para anticuerpos antinucleares y anti-ADN.
For this analysis, the reference ranges of C3 and C4 from The Ohio State University Clinical Laboratory were used to identify the lower limits of normal, and the presence or absence of ADNA was determined by a Crithidia luciliae
Three clinical laboratory methods are commonly used for the determination and quantification of anti-dsDNA: Crithidia luciliae indirect immunofluorescence, ELISA, and RIA (the Farr assay).
The binding of antihistone antibodies to crithidia luciliae kinetoplasts is growth cycle-dependent.
Samples showing homogeneous staining patterns were reevaluated using the Crithidia luciliae
substrate (ImmunoConcepts), which specifically detects antibodies to double-stranded DNA (dsDNA), and by enzymelinked immunosorbent assay (ELISA) to detect antibodies to chromatin (INOVA Diagnostics, San Diego, CA), both according to the manufacturers' instructions.
Anti-dsDNA detection: Three main methods are available for the detection of anti-dsDNA which are the Crithidia luciliae
immunofluorescence (CLIF) method, Farr assay, and ELISA.
Ideal for screening or simultaneous follow up titration of positive ANA sera, the NOVA Lite Hep2k slide contains optimally fixed Hep-2k cells and Crithidia luciliae
together in the same well.