Although punicalagin has not been synthesized yet, the successful preparative separation of punicalagin from crude ethanol extract of pomegranate by high-speed countercurrent chromatography
has been described in reported paper, in which 105 mg of punicalagin was separated from about a 350 mg amount of the crude extract with high purity (Lu et al.
Further examples of topics presented include separation of alkaloids by countercurrent chromatography
, application of capillary electrochromatography to biopolymers and pharmaceuticals, concentration of dilute colloidal samples by field- flow fractionation, laser-induced fluorescence detection for capillary electrophoresis, mass transfer between phases, molecularly imprinted polymers for affinity chromatography, metal-ion enrichment by countercurrent chromatography
, photodiode-array detection, rotation locular countercurrent chromatography
, quantitation by external and internal standards, systematic selection of solvent systems for high speed countercurrent chromatography
, and cold wall effects in thermal field-flow fractionation.
The two-year Small Business Innovative Research (SBIR) grant is WTI's second from the NIH, and will allow the firm to adapt its proprietary technologies to existing chemical separation techniques, namely countercurrent chromatography
(CCC) making this process dramatically easier and less costly.