BSA concentrations were obtained from the Bradford method relying on the binding of the dye Coomassie Blue
G250 to protein , The absorbance at 595 nm was determined by a UV-vis spectrophotometer (UV-1601, Shimadzu, Japan).
Then Neuhoff's colloidal Coomassie blue
G-250 stain was carried out according to the method described by Candiano et al.
and stained in the same solution containing 2% Coomassie Blue
R250 until the bands appeared.
For each gel, an identical gel was run in parallel and subjected to Coomassie blue
After the gels had run to completion, students carefully removed the gels and proceeded to add a Coomassie blue
Following electrophoresis, the gel was stained overnight with Coomassie Blue
R-250 and then destained in the same solution.
Approximately 10-[micro]g samples from hypobranchial glands were loaded and separated by electrophoresis in 2 similar gels, one of which was stained with Coomassie Blue
e permaneceram em solucao contendo 2% Coomassie Blue
R250 ate aparecerem as bandas de proteina.
We used SDS polyacrylamide gel electrophoresis coupled with Coomassie blue
staining to analyze the production and purification of the recombinant proteins (Figure 1, panel A).
With time, Coomassie Blue
staining has become the most widely accepted method for detecting proteins in gels.
Total protein determination in urine: elimination of a differential response between the Coomassie blue
and pyrogallol red protein dye-binding assays.
Gels were stained with Coomassie Blue
for 30 minutes, followed by 1 hour of destaining.