Rolling circle replication in solution was developed to form billions of discrete DNA nanoballs (DNBs), each comprising a concatemer
with approximately 500 copies of the original circle.
However, deletions extending into regions adjacent to the transgene concatemer
are not detected, as two intact cos sites are required for the packaging of a single [lambda] vector.
sequences were analyzed by using SAGE software (version 4.
With the magnetic induction intensity of external magnetic field increasing, the linear extent of flocculation grows obviously by developing from dimers or triplets into concatemers
with the occurrence of branched chains.
double dagger]) adk 387 1 0 argA 357 1 0 aroA 354 1 0 glnA 336 3 0 thrA 339 3 0 tmk 372 4 0 trpE 465 2 0 Concatemers
2,610 6 0 MLST ([paragraph]) inv 603 2 0 yopE 540 3 0 yadA 651 3 6 Concatemers
1,794 3 6 virulence genes (#) Concatemers
4,404 6 6 MVLST ** Target Polymorphic Substitutions, no.
Briefly, DNA fragments were repaired to blunt-ended DNA by fill-in and exonuclease after A-tailing was conducted to prevent the formation of adapters, dimers, and concatemers
A ladder ofbacteriophage lambda concatemers
(New England Biolabs) was used as molecular weight markers.
of the e phage DNA ladder (Promega, Madison, WI, USA) starting at 48.
Lambda DNA concatemers
(New England BioLabs) were used as molecular size markers.
In addition, the PCR products were also analyzed by agarose gel electrophoresis to test for concatemers
and the correct product size of 425 bp.
of lambda DNA were used as molecular size markers (BioRad, Veenendaal, the Netherlands).
were run as molecular weight markers (Roche).