cloning vector

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clon·ing vec·tor

an autonomously replicating plasmid or phage with regions that are not essential for its propagation in bacteria and into which foreign DNA can be inserted; this foreign DNA is replicated and propagated as if it were a normal component of the vector.
A recombinant DNA molecule containing sequences from 2 or more sources—e.g., plasmids, bacteriophage, cosmids—into which foreign DNA—40+ kb—may be inserted for cloning; a DNA sequence capable of self-replication in a suitable host cell, which has site(s) for inserting DNA fragments by recombinant DNA techniques, and genetic markers that allow selection for the vector in a host cell; CVs usually have a selective marker—e.g., for antibiotic resistance—and a site for cleavage by a restriction endonuclease

clon·ing vec·tor

(klōn'ing vek'tŏr)
An autonomously replicating plasmid or phage with regions that are not essential for its propagation in bacteria and into which foreign DNA can be inserted; this foreign DNA is replicated and propagated as if it were a normal component of the vector.

cloning vector

see VECTOR.

vector

1. a carrier, especially the animal (usually an arthropod) which transfers an infective agent from one host to another, e.g the tsetse fly, which carries trypanosomes from animals to humans, dogs, bats and other animals that transmit the rabies virus. In molecular biology, a DNA molecule which serves to transfer DNA into a host cell.
2. a quantity possessing magnitude, direction and sense (positivity or negativity).

biological vector
an arthropod vector in whose body the infecting organism develops or multiplies before becoming infective to the recipient individual.
cloning vector
a DNA molecule used to transfer an inserted DNA segment into a host cell. Includes other viruses, phages and bacterial plasmids. Called also cloning vehicle.
mechanical vector
an arthropod vector that transmits the infective organisms from one host to another but is not essential to the life cycle of the parasite.
shuttle v's
vectors which contain both prokaryotic and eukaryotic replication signals, thus allowing replication of the vector in both kinds of cells.
targeting vector
a vector carrying a DNA sequence that is able to take part in a specified chromosomal crossover in the host.
References in periodicals archive ?
Improved M13 phage cloning vectors and host strains: Nucleotide sequences of the M13mp18 and pUC19 vectors".
Two types of gene specific primers for each gene (Table 1) were designed based on cloning vectors used in cloning reactions of TaNAC69-1 and TtNAMB-2 genes in different expression vectors.
2/blunt and pENTR(tm)/D-TOPO(r) cloning vectors which were used for conventional cloning and Gateway cloning studies, respectively.
Sequence analysis of positive colonies showed that an error free full-length TaNAC69-1 and TtNAM-B2 genes were successfully cloned into cloning vectors and used for construction of different plant transformation vectors.
1983) A cDNA cloning vector that permits expression of cDNA inserts in mammalian cells.
asburiae MS7 followed by ligation of DNA fragments into the BamHI-site of cloning vector pGB2 (32).
As indicated above, the minority judgment holds that the use of the patented gene, vectors, and cells should be limited to their use in creating cloning vectors and transgenic plant cells in a laboratory setting.
Invitrogen offers numerous TOPO Cloning vectors for a variety of applications including vectors for expression in bacterial, yeast, insect and mammalian cells.
The goals of research at the Cooperative Research Center for Food Industry Innovation (CRC, University of New South Wales, Biotechnology Department, Sydney NSW 2052, Australia) are to construct a variety of food-grade cloning vectors for use in LAB and to improve the bacteriophage resistance of dairy starter cultures using genetic techniques.
Application of the knowledge generated from this fundamental genetic research is being implemented toward the construction of more sophisticated food-grade cloning vectors.
Several other novel products are designed to serve researchers working in the human genome project, the development of biologicals and other areas of molecular biology where expression systems, cloning vectors, enzymes and related research tools are required.
7% agarose electrophoresis gel and ligated into pCR(r)4-TOPO-TA cloning vector according to the manufacturer's instructions.