yeast artificial chromosome

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yeast artificial chromosome

n. Abbr. YAC
A large sequence of DNA that is artificially inserted into a yeast genome for replication or expression.

yeast artificial chromosome (YAC)

a yeast chromosome used in recombinant DNA procedures. It can carry large segments of foreign DNA.

yeast artificial chromosome (YAC)

CLONING VECTOR based on YEAST chromosome, with a yeast TELOMERE at each end of the linear molecule, a CENTROMERE, a sequence for autonomous REPLICATION, allowing propagation in yeast, a selectable GENETIC MARKER for yeast and a POLYLINKER. YAC vectors typically accommodate DNA fragments up to 600kb in size. However, special types of vector can accept fragments up to 1400kb. The very high capacity of these vectors makes them useful for cloning large inserts in genome sequencing projects.

yeast

a general term including unicellular, nucleated, usually rounded fungi that reproduce by budding; some are fermenters of carbohydrates, and a few are pathogenic for animals. See also mastitis.

yeast artificial chromosome
cloning vectors developed for the cloning of large (200-500 kbp) DNA fragments; YAC libraries permit the cloning of large genes with their flanking regulatory sequences as well as families of contiguous genes. They are difficult to work with and have the further disadvantage that the cloned sequences are unstably retained.
brewer's yeast
Saccharomyces cerevisiae used in brewing beer, making alcoholic liquors, and baking bread. See also dried yeast (below).
dried yeast
dried cells of any suitable strain of Saccharomyces cerevisiae, usually a by-product of the brewing industry; used as a natural source of protein and B-complex vitamins.
yeast two-hybrid system
an experimental technique for identifying genes whose protein product interacts with another particular protein of interest.