The kinetics of the neutral hydrolysis of 4-nitrophenyl chloroacetate was followed spectrophotometrically with a Perkin-Elmer Lambda 2S spectrometer.
The sonolytic degradation of 4-nitrophenol was monitored identically under the same conditions as the hydrolysis of 4-nitrophenyl chloroacetate.
Therefore the rate of sonolytic degradation of 4-nitrophenol was separately studied under the same conditions as used for the hydrolysis of 4-nitrophenol chloroacetate.
Histochemical staining for chloroacetate
esterase (Leder stain), which stains neutrophils and precursors, is usually positive, although sometimes only focally.
Summary: Melamine and sodium chloroacetate were used as raw materials to synthesize a kind of quaternary ammonium salt (QAS) as potential clay swelling inhibitor and water-based drilling fluid additive, and the reaction conditions were screened based on the linear expansion rate of bentonite.
Melamine and sodium chloroacetate were provided by Sinopharm Chemical Reagent Co.
Normal, reactive, and neoplastic mast cells stain with naphthol AS-D chloroacetate
esterase (CAE) and are negative for myeloperoxidase expression.
Myeloblasts (but not erythroblasts) are usually positive for myeloperoxidase (MPO), Sudan Black B, and, often, chloroacetate
Cytochemical staining (Leder stain) showed the blasts were positive for chloroacetate
esterase, whereas the crystals were negative for myeloperoxidase and chloroacetate
The original diagnostic BM aspirate smears and subsequent peripheral blood smears were Wright stained and stained for the presence of the following: myeloperoxidase (MPO), Sudan Black B (SBB), chloroacetate
esterase (CAE), [Alpha]-naphthyl acetate esterase (ANAE), and [Alpha]-naphthyl-butyrate esterase (ANBE).
The BM aspirate smears and touch preparations of the BM core biopsy had been routinely stained with Wright stain and for the presence of MPO, Sudan black B, [Alpha]-naphthyl acetate esterase, [Alpha]-naphthyl butyrate esterase, and chloroacetate
esterase (Sigma Chemical Co, St Louis, Mo) according to kit procedures.
The blasts showed coarse granular staining for periodic acid-Schiff, but failed to stain with Sudan black B and chloroacetate