No attempts were made to antigenically characterize the strain to confirm differences from the Caraparu virus.
Phylogenetic trees based on the S and L gene segments placed the IQT9646 virus among isolates of Caraparu virus, a member of the group C virus serogroup (Figures 2, 3), a pathogen known to cause febrile illness in humans and animals in the Amazon region of Peru (9,11).
These antisera displayed a minimum 4-fold difference in neutralization titers between these viruses, indicating that IQT9646 is serologically distinct from the prototype Caraparu virus.
The strain FSL2923, isolated from a febrile patient residing in Yurimaguas, Peru, in 2006 was found to possess L and S RNA segments closely related to Caraparu virus; the M segment was only [approximately equal to] 75% identical to the M segment of Caraparu virus (11).
Considering our findings with the IQT9646 strain, which, like FSL2923, possesses the S and L RNA segment sequences closely related to Caraparu virus and the M RNA segment sequence derived from an unidentified group C virus, we made genetic comparison and observed that the S, M, and L RNA segments of IQT9646 and FSL2923 shared >98% nucleotide and >99% amino acid sequence homology for all 3 viral RNA segments.
Estao incluidos patogenos virais de significante importancia humana e veterinaria, tais como, Crimean-Congo hemorrhagic fever virus (CCHFV), Rift Valley fever virus (RVFV), Akabane virus (AKAV), Nairobi sheep disease virus (NSDV), Aino virus (AINV) e Peaton virus (PEAV) (4), assim como virus ja isolados na Amazonia Brasileira como: Guaroa virus (GROV), Maguari virus (MAGV), Tacaiuma virus (TCMV), Guama virus (GMAV), Caraparu virus
(CARV), Oropouche virus (OROV), Catu virus (CATUV), Icoaraci virus (ICOV) e Belem virus (BLMV), entre outros (2).
Louis encephalitis virus, Rocio virus, Ilheus virus, West Nile virus, Venezuelan equine encephalitis virus, eastern equine encephalitis virus, Mayaro virus, Trocara virus, Oropouche virus, Caraparu virus
, Murtucu virus, Guaroa virus, hantavirus, Machupo virus, and Tacaribe virus; after the cardiovirus isolation, EMCV antibody also was used.