These results suggest that CRABPs may play an important role in the regulation of intracellular retinoic acid concentrations during adipogenesis.
CRABPs are believed to be involved in modulating intracellular RA concentrations (Ong et al.
In this study, we have cloned the bovine CRABPs and determined CRABPs expression in normal tissues and regulation of CRABPs during adipogenesis using intramuscular fibroblast-like cells to evaluate the importance of CRABPs on adipocyte differentiation in the bovine.
Up-regulation of bovine CRABPs during adipocyte differentiation of intramuscular fibroblast-like cells
To analyze the function of bovine CRABPs during adipocyte differentiation of intramuscular fibroblast-like cells, we isolated bovine intramuscular fibroblast-like cells from bovine sirloin tissues and differentiated to adipocytes by exposure to differentiation medium for 18 days.
To determine the expression of bovine CRABPs mRNA during adipocyte differentiation, we performed real-time RT-PCR analysis using total RNA over the period day 0-day 18.