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It was negative for CK20, calretinin, vemintin and prostate-specific antigen.
Immunophenotyping was positive for pankeratin, vimentin, CD20, P504S and prostate-specific antigen (PSA), and negative for CK7, CK20 and prostate specific acid phosphatase (PSAP).
Paraffin-embedded tissue blocks representative of the tumors were retrieved, and immunostaining for AE1/AE3 (Dako, Carpinteria, California), cytokeratin (CK) 7 (Dako), CK20 (Dako), CD56 (Ventana Medical Systems, Tucson, Arizona), synaptophysin (Dako), chromogranin (Dako), and thyroid transcription factor 1 (Dako) was performed using commercially available antibodies.
However, with regards to terminal differentiation, there was a remarkable difference in favour of the organ-specific BSCs equivalents, which showed minimal expression of CK14 and obvious expression of CK20, while DF-SMCs equivalents revealed remarkable expression of CK14, mainly in the basal cells with no expression of CK20.
Although a small chance remains that the site of origin was carcinoma of the urothelium of the renal pelvis, given the concomitant extensive pulmonary lesions and positive CK20 on immunohistochemistry, squamous cell carcinoma of the lung with renal metastasis is much more likely.
Both lesions were positive for CA19-9, CEA, CK20, and CK7 immunohistochemical stains.
The primary antibodies used were those against CK7 (Dako; Clone OV-TL12/30; 1: 600), CK20 (Dako; Clone Ks20.
Merkel cell carcinoma reacts positively for neuroendocrine markers such as synaptophysin and chromogranin, showing a characteristic perinuclear dot-like staining pattern with pan-cytokeratin and CK20 immunohistochemistry (figure 2).
There were no correlations between loss of PTEN expression and the histologic subtype, tumor differentiation, presence of adenomatous component, resection margin status, pT stages, adjuvant therapy status, or expression of cytokeratin (CK) 7, CK20, and CDX2.
encoding CK18, CK19, CK20, MUC1, prostate-specific antigen, and carcinoembryonic antigen) are also produced at low concentrations in normal blood and BM cells (17, 18).
In immunochemistry, CK20 was strongly positive, SI00 was focally positive, and EMA was positive, while synaptophysin, chromogranin, vimentin, CD3, CD20, as well as CD45, and CD99 were all negative.
But immunohistochemical markers such as ER, PR, gross cystic disease fluid protein (GCDFP-15), and differential expression of CK7 and CK20 can facilitate an accurate diagnosis as seen in the present report.
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- Civatte bodies
- Civatte body
- Civatte, Achille
- civil action
- civil code
- civil commitment
- Civil Contingencies Act 2004
- Civil Defense Model
- civil law
- Civil Partnership Act
- civil surgeon
- Civilian Health and Medical Program of the Uniformed Services
- Civilian Health and Medical Programs for Uniformed Services
- Civinini, Filippo
- CK isoenzyme fraction
- CK syndrome
- CKD-EPI creatinine equation
- CL domain
- CL lead
- Cl-/HCO3- exchanger
- Clado anastomosis
- Clado ligament
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- Clado, Spiro
- Clado’s ligament
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