3 Immunohistochemically, epithelium shows positive expressions of cytokeratins (CK), especially CK5, CK14
, and CK19, and mesenchymal tumor cells show positive expression of nestin.
Basal cell carcinoma displays a profile of cytokeratin expression similar to follicular germinative cells, characterized by CK5/6 and CK14
expression and the absence of CK20.
Immunohistochemical analyses included staining with Ki-67, p53, epidermal growth factor receptor (EGFR), cytokeratin (CK) 8, CK14
, CK17, and CK18.
Immunhistochemical testing can be helpful to differentiate between thymoma and thymic lymphoma by labeling cytokeratins CK14
and CK18, as well as showing positive results for proliferating cell nuclear antigen in cases of thymomas.
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Para el analisis de los casos, se agruparon los 22 marcadores utilizados en cuatro grupos: a) marcadores de la clasificacion molecular (ER, PR y HER2), b) marcadores de proliferacion celular y otros (EGFR, C-Kit, p63, survivina, ALDH1, CD10 y Ki-67), c) marcadores de filamentos intermedios (Ck5/6, CK8/18, CK14
, CK17, CK APM y vimentina), d) marcadores de adhesion celular y citoesqueleto (cadherina E, cadherina P, p120, actina de musculo liso, alfa actina y calponina).
However, with regards to terminal differentiation, there was a remarkable difference in favour of the organ-specific BSCs equivalents, which showed minimal expression of CK14
and obvious expression of CK20, while DF-SMCs equivalents revealed remarkable expression of CK14
, mainly in the basal cells with no expression of CK20.
In "triple negative" cases, additional immunohistochemical analyses were performed, using several markers that have been reported as useful in defining the basal-like phenotype, including EGFR (3C6, prediluted, Ventana Medical Systems, Tucson, AZ), CK5/6 (D5 & 16B4, prediluted, Ventana Medical Systems, Tucson, AZ) and CK14
(LL002, prediluted, Ventana Medical Systems, Tucson, AZ).
36] found frequent positivity for SMA and CK14
, as well as immunoreactivity for S100 protein, p63, maspin and p-cadherin.
Results: The positive expression rates of p63 CK5/6 CK14
and CK17 in the elderly group were significantly higher than those of the youth group (P less than 0.
, EGFR, 34[beta]E12 used to identifying subgroups of basal-like breast cancers.
Briefly, the tissue was homogenized in 1 mL DNAzol using Precellys 24 bead mill (Bertin, Montigny-le-Bretonneux, France), using ceramic CK14
beads at 5,000g for 10 sec.