However, with regards to terminal differentiation, there was a remarkable difference in favour of the organ-specific BSCs equivalents, which showed minimal expression of CK14
and obvious expression of CK20, while DF-SMCs equivalents revealed remarkable expression of CK14
, mainly in the basal cells with no expression of CK20.
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Immunohistochemically, the neoplastic cells were positive for cytokeratin AE1 / AE3, CK14
, smooth-muscle actin, widespread vimentin, focal S-100 protein, focal glial fibrillary acidic protein, and focal epithelial membrane antigen.
, EGFR, 34[beta]E12 used to identifying subgroups of basal-like breast cancers.
9) Cytokeratin 14 (CK14
) and cytokeratin 17 (CK17) have both been reported as positive immunostains for BCC, (10) although CK14
has not been studied in BCCm.
Briefly, the tissue was homogenized in 1 mL DNAzol using Precellys 24 bead mill (Bertin, Montigny-le-Bretonneux, France), using ceramic CK14
beads at 5,000g for 10 sec.
There was no staining for AE1 AE3, pancytokeratin, H-caldesmon, myogenin, CK 5/6, CK14
, p63, 34BE12, CD34, CK7, CD56, EMA, S100, melan A, c-KIT and CD99.
Yanagita et al (52) created an immunostaining method using a 4-antibody cocktail of TTF-1, napsin A, p63, and CK14
with high specificity and sensitivity for differentiating ACAs and SCCs of the lung.
The monoclonal antibody 34[beta]12 targets cytoplasmic highmolecular-weight cytokeratin filaments in basal cells of the benign prostatic glands, namely CK1, CK5, CK10, and CK14
Antibodies to uroplakin III (UROIII), S100P, and GATA-3 have been shown to be urothelial-associated markers, while expression of CK14
and desmoglein-3 has been associated with squamous differentiation.
The basal cytokeratin stains (CK5, CK14
, and CK17), recently shown to be expressed in the basal phenotype of breast carcinoma, were not present in most other types of PBC.
Antibodies to a number of basal-type and highmolecular-weight cytokeratins, including cytokeratin (CK) 1, CK5, CK5/6, CK10, CK14
, and CK17, react variably with breast MECs.