was performed on 19 cases and showed characteristic dot like staining.
Mucosal melanoma is usually positive for S100, HMB-45, Melan-A, and melanogenesis-associated transcription factor (MITF), and negative for cytokeratins, CD99
, desmin, myogenin, and CD45.
Immunohistochemically, the tumor cells were positive for vimentin, sex determining region of Y chromosome-related high-mobility group box gene-9 (SOX-9), S-100, P16, murine double minute 2 (MDM2), and cyclin-dependent kinase 4 (CDK4) and negative for pan-cytokeratin (PCK), epithelial membrane antigen (EMA), smooth muscle actin (SMA), desmin, myogenin, cluster of differentiation 10 (CD10), CD31, CD34, caldesmon, CD99
, beta-catenin, signal transducer, and activator of transcription 6 (STAT-6).
It was consistent with PNET being positive for vimentin, CD99
and FLI-1 (friend leukaemia integration-1) and negative for desmin, mesothelin and cytokeratin.
Sonrasinda patolojik tani icin yapilan osteocut ile kemik biyopsisinden elde edilen materyalin immunohistokimyasal incelenmesinde CD 45, EMA, Tdt, S-100, Pan-CK, Myogenin tumor markerlari negatif gelirken Ewing sarkomu icin spesifik olan Vimentin ve CD99
pozitif olarak saptandi.
The mass was negative for CD99
, cytokeratin, smooth muscle actin, muscle-specific actin, S100, desmin, LCA, CD3, CD20, CD31, and myoglobin.
Additionally these lesions usually stain for CD34, desmin, BCL2, CD99
, CD10 and WT1.
Immunohistochemistry showed strong membranous positivity with CD99
and some positivity with CD56 and synaptophysin.
Immunohistochemistry: Collagen type IV, vimentin, nuclear MDM2 positivity, BCL-2, CD99
, CD34 focal positivity.
immunostain positivity plays its utmost diagnostic role in these tumors.
All other tested markers (TCA, CD3, CD79a, chromogranin A, synaptophysin, myogenin, desmin, S-100 protein, TdT, LMP1, CD99
, TTF-1 and CK-14) were negative, except for a weak expression of neuron-specific enolase (NSE).
These molecules include platelet/EC adhesion molecule-1 (PECAM-1), CD99
, junctional adhesion molecules A and C (JAM-A and JAM-C), and JAM-like protein (JAML) .