Ademas, pueden favorecer la maduracion de las celulas dendriticas (DC, por su sigla en ingles) mediante la produccion de citocinas, incluyendo IL-12p70, asi como el aumento en la expresion de CD80, CD83
, CD86 y CMH-II (23).
Cada una de las poblaciones se evaluaron para la expresion de CD14: HLADR y la coexpresion de CD83
y CD86, por citometria de flujo.
2] qPCR Primer Assay for human CD83
and as housekeeping genes [RT.
27-Hydroxycholesterol (27OHChol) also rapidly induces the differentiation of THP1 cells into mDCs  with high expression of mDC-specific markers such as CD80, CD83
, CD86, and CD88.
Morphological changes and the upregulation of costimulatory molecules (CD86, CD83
, CD80, and CD40) during DC maturation are essential for the activation and recruitment of T cells or other immune effectors.
Transcription analysis of 44 CD genes was also carried out, indicating 24 CD genes (CD3E, CD244, CD69, CD247, CD40L, CD38, CD5L, CD44, CD5, CD83
, CD163L1, CD36, CD14, CD28, CD200R1A, CD40, CD59, CD200, CD4, CD2, CD72, CD180, CD79B, and CD93) to be markedly increased by 2.
Alcohol also suppressed expression of the co-stimulatory molecule CD83
during DC maturation, which may attenuate the ability of DCs to initiate T-cell expansion (Siggins et al.
DC-SIGN-positive cells appear to be immature dendritic cells in that they did not express the dendritic cell maturation molecule CD83
To assess the maturation status of lung CD11c+CD14-mDCs after IAV infection, the co-stimulatory markers such as HLA-DR, CD80, CD83
, and CD86 were analyzed by FACS.
Expression of the CD83
transmembrane domain in DCs interferes with modulation of MHC II and B7.
However, no significant difference was observed in cell proliferation, morphology and phenotype between group A and B, but the mRNA expression levels of surface marker genes, MHC-II, CD40, CD80, CD83
and CD86 were up regulated (<0.
Expression of CCR6 and CD83
by cytokine-activated human neutrophils.