These cells expressed CD90, CD44, CD105, and CD73
but not CD45, CD11b, CD19, HLA-DR, and CD34 [Figure 2]b.
They found what they were looking for in the so-called CD73
After 3 passages the mesenchymal entity of BMMSCs was proved with special markers (expression of CD44 and CD73
and non-expression of CD45 and CD11b) using flow cytometry and their multi-potential entity was proved by their differentiation into osteogenic and adipogenic cells within 21 days (Soleimani & Nadri, 2009).
Third-passage DFSCs were incubated with the antibodies of positive markers for MSCs of human CD29 allophycocyanin (APC), CD73
phycoerythrin (PE), CD105 PE, and CD146 fluorescein isothiocyanate (FITC) and with the antibodies of negative markers of CD14 PE, CD34 FITC, CD45 APC, and HLA-DR PE (BD Biosciences, USA) for 15 min at room temperature in the dark.
The immunophenotypic profile was analyzed using the antibodies CD14, CD34, CD44, CD45, CD73
, CD90, CD146, CD184, Stro-1 and HLA-DR (Becton Dickinson, USA) conjugated with FITC or PE fluorochrome.
MSCs are characterized on the basis of surface expression markers as CD73
, CD90, and CD105 positive, and CD34 and CD45 negative .
Furthermore, inhalational anesthetics produce and promote anti-inflammatory mediators important for renal protection, including TGF-[beta]1 release, CD73
activation, and adenosine generation (20-22).
, CD90 and CD105 (DE MATTOS CARVALHO et al.
High concentration of adenosine in human visceral leishmaniasis despite increased ADA and decreased CD73
The proinflammatory signaling of extracellular ATP is terminated by nucleotidases active in the interstitial fluid and the membrane-bound ectonucleotidases, CD39 and CD73
The criteria of the Mesenchymal and Tissue Stem Cell Committee of the International Society for Cellular Therapy reported that stem cells that expressing the markers of CD44, CD90, CD73
and CD105 are considered mesenchymal and those that express markers of CD19, CD14, CD34 or CD45 are regarded hematopoietic stem cells.
Hawrylowicz, "1[alpha],25-dihydroxyvitamin D3 acts via transforming growth factor-[beta] to up-regulate expression of immunosuppressive CD73
on human [CD4.