containing a tetrameric antibody complex that recognizes both the CD66b
antigen and the dextran of iron-coated nanoparticles.
Neutrophil infiltration was measured using a monoclonal-antibody stain against CD66b
, a granulocyte membrane antigen (about 96% of granulocytes in blood vessels are neutrophils).
Selectin "shedding" occurs because infected cells degranulate, including an EDTA-inhibitable sheddase (metalloprotease), [beta]2-integrins, CD66b
, and other inflammatory components such as matrix metalloproteases, which includes gelatinase (MMP9) (38,39).
Szekanecz Z, Haines GK, Harlow LA, et al: Increased synovial expression of the adhesion molecules CD66a, CD66b
and CD31 in rheumatoid and osteoarthritis.
We used 3-color analyses to identify granulocytes as sidescatter high, CD66b
high; monocytes as side-scatter intermediate, CD33 high; and T lymphocytes as sidescatter low, CD2 high.